Transfusion compatibility cross-matching, agglutination testing, and hemodynamics volume estimation.
ABO & Rh Antigen System: Karl Landsteiner discovered erythrocyte surface carbohydrates (A, B agglutinogens) and IgM antibodies (anti-A, anti-B). The Rh(D) polypeptide determines positive ($+$) vs negative ($-$) status.
Transfusion Rules: $O^-$ red blood cells lack A, B, and Rh antigens, functioning as the Universal Red Cell Donor. $AB^+$ patients lack serum anti-A, anti-B, and anti-Rh antibodies, acting as Universal Recipients.
Blood Volume Dynamics: Total adult blood volume is approximately $70\text{ mL/kg}$ body mass ($55\%$ plasma, $45\%$ packed erythrocytes / hematocrit, $<1\%$ buffy coat leukocytes).
Operating Instructions: Select Donor and Recipient blood groups to test serum agglutination clumping and verify transfusion safety.
No hemolytic agglutination detected between donor erythrocytes and recipient serum antibodies.
| Recipient \\ Donor | O- | O+ | A- | A+ | B- | B+ | AB- | AB+ |
|---|